cultrex basement membrane extract, pathclear Search Results


cultrex  (R&D Systems)
95
R&D Systems cultrex
VT107 alters the capacity of extracellular vesicles isolated from TNFα-treated U87 glioblastoma cells to trigger in vitro mesenchymal stem cells vasculogenic mimicry. ( A ) Representative phase contrast images were taken to monitor the formation of mesenchymal stem/stromal <t>cells</t> <t>capillary-like</t> structures for up to 6 h (upper panels) and were analyzed via Wimasis (lower panels). ( B ) Quantification from Wimasis analysis of the total loops over time. ( C ) <t>Cultrex</t> was depleted from soluble growth factors, then used to monitor the capacity to re-induce capillary-like structure formation upon 6 h (upper panels). The structures were analyzed via Wimasis (lower panels). Total loops were quantified to assess in vitro VM. ( D ) The impact of serum-free media (closed triangles), EVs (open circles) and of serum-enriched media (closed circles), or ( E ) the impact of EVs isolated from control, 30 ng/ml TNFα-, 1 µM VT107-, or TNFα/VT107-treated cells, was assessed on in vitro VM
Cultrex, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+PathClear/pmc12523088-78-6-8
Average 95 stars, based on 1 article reviews
cultrex - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
cultrex pathclear basement membrane extract bme gels  (R&D Systems)
94
R&D Systems cultrex pathclear basement membrane extract bme gels
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Pathclear Basement Membrane Extract Bme Gels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+PathClear/pmc06340043-408-14-21
Average 94 stars, based on 1 article reviews
cultrex pathclear basement membrane extract bme gels - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
matrigel  (R&D Systems)
95
R&D Systems matrigel
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Matrigel, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+Pathclear/pm25176450-54-23-25
Average 95 stars, based on 1 article reviews
matrigel - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
cultrex basement membrane extract  (R&D Systems)
95
R&D Systems cultrex basement membrane extract
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+PathClear/bio_rxiv__2025__09__18__677171-68-16-21
Average 95 stars, based on 1 article reviews
cultrex basement membrane extract - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
matrigel bme type 3  (R&D Systems)
93
R&D Systems matrigel bme type 3
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Matrigel Bme Type 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+Type+3%2C+Pathclear/pm40722609-145-20-27
Average 93 stars, based on 1 article reviews
matrigel bme type 3 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
cultrex pathclear basement membrane extract  (R&D Systems)
95
R&D Systems cultrex pathclear basement membrane extract
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Pathclear Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+PathClear/pmc08602366-32-0-23
Average 95 stars, based on 1 article reviews
cultrex pathclear basement membrane extract - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
basement membrane extract  (R&D Systems)
94
R&D Systems basement membrane extract
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+Pathclear+(w%2F+Phenol+Red)/pm41801228-260-19-25
Average 94 stars, based on 1 article reviews
basement membrane extract - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
cultrex bme  (R&D Systems)
94
R&D Systems cultrex bme
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+Type+3%2C+Pathclear/bio_rxiv__2022__07__26__501603-314-26-28
Average 94 stars, based on 1 article reviews
cultrex bme - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
cultrex bme type 3  (R&D Systems)
94
R&D Systems cultrex bme type 3
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Bme Type 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+basement+membrane+extract%2C+pathclear/Cultrex+Basement+Membrane+Extract%2C+Type+3%2C+Pathclear/pmc08941982-143-0-4
Average 94 stars, based on 1 article reviews
cultrex bme type 3 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


VT107 alters the capacity of extracellular vesicles isolated from TNFα-treated U87 glioblastoma cells to trigger in vitro mesenchymal stem cells vasculogenic mimicry. ( A ) Representative phase contrast images were taken to monitor the formation of mesenchymal stem/stromal cells capillary-like structures for up to 6 h (upper panels) and were analyzed via Wimasis (lower panels). ( B ) Quantification from Wimasis analysis of the total loops over time. ( C ) Cultrex was depleted from soluble growth factors, then used to monitor the capacity to re-induce capillary-like structure formation upon 6 h (upper panels). The structures were analyzed via Wimasis (lower panels). Total loops were quantified to assess in vitro VM. ( D ) The impact of serum-free media (closed triangles), EVs (open circles) and of serum-enriched media (closed circles), or ( E ) the impact of EVs isolated from control, 30 ng/ml TNFα-, 1 µM VT107-, or TNFα/VT107-treated cells, was assessed on in vitro VM

Journal: Cell Communication and Signaling : CCS

Article Title: Hippo pathway suppression reprograms TNFα-primed glioblastoma extracellular vesicles transcripts cargo to drive mesenchymal stem/stromal cells vasculogenic mimicry

doi: 10.1186/s12964-025-02401-x

Figure Lengend Snippet: VT107 alters the capacity of extracellular vesicles isolated from TNFα-treated U87 glioblastoma cells to trigger in vitro mesenchymal stem cells vasculogenic mimicry. ( A ) Representative phase contrast images were taken to monitor the formation of mesenchymal stem/stromal cells capillary-like structures for up to 6 h (upper panels) and were analyzed via Wimasis (lower panels). ( B ) Quantification from Wimasis analysis of the total loops over time. ( C ) Cultrex was depleted from soluble growth factors, then used to monitor the capacity to re-induce capillary-like structure formation upon 6 h (upper panels). The structures were analyzed via Wimasis (lower panels). Total loops were quantified to assess in vitro VM. ( D ) The impact of serum-free media (closed triangles), EVs (open circles) and of serum-enriched media (closed circles), or ( E ) the impact of EVs isolated from control, 30 ng/ml TNFα-, 1 µM VT107-, or TNFα/VT107-treated cells, was assessed on in vitro VM

Article Snippet: VM was assessed in vitro using Cultrex (3432-010-01, R&D Systems) to monitor capillary-like structures formation [ , ].

Techniques: Isolation, In Vitro, Control

Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on Cultrex PathClear basement membrane extract (BME) gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.

Journal: Journal of Virology

Article Title: Development of a Primary Human Cell Model for the Study of Human Cytomegalovirus Replication and Spread within Salivary Epithelium

doi: 10.1128/JVI.01608-18

Figure Lengend Snippet: Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on Cultrex PathClear basement membrane extract (BME) gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.

Article Snippet: Following digestion and lysis of red blood cells, the salivary cells were plated on Cultrex PathClear basement membrane extract (BME) gels (R&D Systems) and cultured in BEGM growth medium (containing the BEGM bullet kit and 4% charcoal-stripped fetal bovine serum [FBS]), provided by Lonza.

Techniques: Isolation, Cell Culture, Membrane, Infection, Expressing, Gene Expression, In Vitro, In Vivo